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Image Search Results
Journal: bioRxiv
Article Title: NT5DC2 downregulation suppresses monoamine oxidase activity and increases catecholamine levels in PC12D cells
doi: 10.1101/2025.05.07.651779
Figure Lengend Snippet: A) Binding procedure . Anti-DYKDDDDK-tag antibody magnetic beads were reacted with 60 µL of cell lysate (1 mg/mL protein) expressing NT5DC2-tag or control-tag at 4°C for 2 h to bind NT5DC2-tag-binding proteins. After washing with PBS/Ca/Mg, 15 µL of SDS/2-mercaptoethanol sample buffer was added, followed by incubation at 80°C for 20 min. The resulting solution was analyzed as the “binding fraction.” B) Detection of MAO A . Proteins in “binding fraction” were analyzed via western blotting using SDS-PAGE and Phos-tag SDS-PAGE.
Article Snippet: The pCMV6 vector, which contained rat NT5DC2 cDNA and cDNA sequences for the Myc tag and DYKDDDDK tag on its 3’ side (
Techniques: Binding Assay, Magnetic Beads, Expressing, Control, Incubation, Western Blot, SDS Page